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ACAT2, Recombinant, Human (Acetyl-CoA Acetyltransferase, Cytosolic, Acetyl-CoA Transferase-like Protein, Cytosolic Acetoacetyl-CoA Thiolase, ACTL)

Cat no: A0220-01B

ACAT2, Recombinant, Human (Acetyl-CoA Acetyltransferase, Cytosolic, Acetyl-CoA Transferase-like Protein, Cytosolic Acetoacetyl-CoA Thiolase, ACTL)

ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. \n\nSource:\nRecombinant corresponding to aa1-397 of human ACAT2 protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF \n\nMolecular Weight: 45.4kD (433aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0220-01B

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.

Purity

>95% (SDS-PAGE).

References

1. Alger HM., et al. (2010) J Biol Chem. 285(19):14267-74. 2. Bryleva EY., et al. (2010) Proc Natl Acad Sci USA. 107(7):3081-6.

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