AcCoA Acetyltransferase (ACAT, Acetyl Coenzyme A Acetyltransferase, Aceto-cetyl Coenzyme A Thiolase), CT

Triacylglycerol hydrolase is a microsomal enzyme that hydrolyzes stored cytoplasmic triacylglycerol in the liver and participates in the lipolysis/re-esterification cycle during the assembly of very-low-density lipoproteins. Expression of human TGH in Escherichia coli yields a protein without enzymatic activity, which suggests that posttranslational processing is necessary for the catalytic activity (1). ACAT esterifies free cholesterol and stores cholesteryl esters in lipid droplets. Macrophage ACAT1 deficiency results in increased atherosclerotic lesion area in \nhyperlipidemic mice via disrupted cholesterol efflux, increased lipoprotein uptake, accumulation of intracellular vesicles, and accelerated apoptosis (2). Data show that macrophage ACAT1 influences the efflux of both cellular and lipoprotein-derived cholesterol and propose a pathway for the pro-atherogenic transformation of ACAT1(-/-) macrophages.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 1:10,000 \nImmunohistochemistry: 1: 250-500 \nImmunocytochemistry: 1:50-100 \nImmunoprecipitation: 1:100 \nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A0220-01H

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Serum

Isotype

IgG

References

1. Alam M, et al. Biochemistry 41(21): 6679-87, 2002. 2. Dove DE, et al. Atherosclerosis 186(2): 267-74, 2006. 3. Sone DE, et al. Arterioscler Thromb Vasc Biol 25(1): 128-34, 2005.

Additional Info

Recognizes human ACAT