AcCoA Carboxylase 1, Human, Control Peptide (ACC-1, Acetyl Coenzyme A Carboxylase 1)

Control Peptide for A0225-01A (affinity purified antibody) and A0225-01B (antiserum).\n\nIn cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC-alpha is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA, the rate-limiting step in the biogenesis of LCFA-CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase (catalytic activity). Two variants of ACC-1 have been described. One with 8 additional amino acids commencing at Pro-1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by camp-dependent kinase. The two ACC1 isoform are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA: 50-100ng of peptide per well.\nAntibody Blocking: 5-10ug per 1ug A0225-01A (affinity purified antibody) or per 1ul A0225-01B (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0225-01

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Purity

Highly purified

References

1. Munday, M.R., et al., (1988) Eur. J. Biochem. 175: 331. 2. Abu-Elheiga, et al., (2000) PNAS 97: 1444. 3. Lee, et al., (2001) J. Biol. Chem. 276: 2,576. 3. Abu-Elheiga, et al., (1997) J. Biol. Chem. 272: 10,699. 4. Hoppel, et al., (2001) ABB 392: 321. 5. Fraser & Zammit, (1998) Biochem. J. 329: 225. 6. Stapleton, et al., (1996) J. Biol. Chem. 271: 611. 7. Mitchelhill, et al., (1997) J. Biol. Chem. 272: 24,475.

Additional Info

Synthetic peptide 15aa sequence mapping near the N-terminus of Human ACC-1. No significant sequence homology is seen with ACC-2 or any other protein. Species Sequence Homology: Rat, human, bovine, sheep: 100%; chicken: 86%.