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AcCoA Carboxylase beta, C-terminal HIS-Tag, Recombinant, Human (ACACB, acetyl-Coenzyme A carboxylase beta, ACC2, ACCB, ACC-beta, Acetyl-CoA carboxylase 2, HACC275)

Cat no: A0225-08

AcCoA Carboxylase beta, C-terminal HIS-Tag, Recombinant, Human (ACACB, acetyl-Coenzyme A carboxylase beta, ACC2, ACCB, ACC-beta, Acetyl-CoA carboxylase 2, HACC275)

Source:\nRecombinant corresponding to full length human ACC2 with C-terminal His tag expressed in Baculovirus infected Sf9 cell (NM_001093).\n\nSpecific Activity: \n~20 pmoles/min/ug. A 25ul ACC2 reaction is conducted in a buffer containing 30mM HEPES, pH 7.4, 2mM MgCl2, 2mM potassium citrate, 1mM DTT, 100uM ATP, 12mM NaHCO3 and 20uM substrate acetyl-Co-A at RT for 45min ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform.\n\nApplications: \nSuitable for use inthe study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -70 degrees C. Aliquots are stable for at least 6 months at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0225-08

Size

10ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 3mM DTT, 0.05% Tween-20, 20% glycerol.

Purity

~70% Purified.

References

1. Locke G.A. et al., Arch. Biochem. Biophys. 475(1), 72-79 (2008). 2 Abu-Elheiga L.. et al., Proc. Natl. Acad. Sci.U.S.A. 97(4), 1444-1449 (2000).

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Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

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