Acetate Assay Kit, BioAssay(TM)

Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibers.\n\nA0504-85 uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.\n\nKey Features:\nUse as little as 10ul samples. Detection range: 0.20-20mM acetate for colorimetric assays and 0.13-2mM for fluorimetric assays.\n\nApplications:\nDirect Assays: Acetate in biological samples such as serum/plasma, in food, agriculture and environmental samples.\nDrug Discovery/Pharmacology: Effects of drugs on acetate metabolism.\n\nKit Components:\nA0504-85A: Assay Buffer, 1x25ml \n*A0504-85B: Enzyme A, 1x1vial\n*A0504-85C: Enzyme B, 1x1vial\nA0504-85D: Developer, 1x1ml\nA0504-85E: Dye Reagent, 1x120ul \nA0504-85F: ATP, 1x120ul \nA0504-85G: Standard, 200mM, 1x1ml \n\nStorage and Stability:\nStore all components at -20 degrees C. Kit is stable for 6 months. *A0504-85B and *A0504-85C after reconstitution stable for four weeks at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n \nPrecautions: \nReagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.

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SPECIFICATIONS

Catalog Number

A0504-85

Size

1Kit

References

1. Laker MF, Mansell MA (1978). Measurement of acetate in aqueous solutions and plasma by gas phase chromatography using a porous polymer stationary phase. Ann Clin Biochem. 15(4):228-32. 2. Desch G, Descomps B (1977). Rapid gas chromatographic method for determination of acetate in human plasma and hemodialysis baths. Clin Chim Acta. 76(2):193-204. 3. Rocchiccioli F. et al (1989). Capillary gas-liquid chromatographic/ mass spectrometric measurement of plasma acetate content and (2- 13C) acetate enrichment. Biomed Environ Mass Spectrom. 18:816-9.