Acetate BioAssay(TM) Kit (Colorimetric)

Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for post-translational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine. \n\nSample Type:\nSerum, plasma, animal tissues (liver, kidney, muscle, heart etc), cell culture (adherent or suspension cells) and food.\n\nIntended Use:\nThe kit can detect less than 20uM of acetate in various samples. Used for measurement of acetate in various tissues/cells and analysis of metabolism and cell signaling in various cells.\n\nTest Principle:\nIn the Acetate BioAssay(TM) Kit, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix & Acetate Substrate Mix. The intermediate reduces a colorless probe to a colored product with strong absorbance at 450nm. The Acetate Colorimetric Assay Kit is rapid, simple & sensitive. It can detect less than 20uM acetate in various samples.\n\nKit Components:\nAcetate Assay Buffer, 1x27ml\nAcetate Enzyme Mix, lyophilized, Ix vial\nATP, lyophilized, 1x vial\nAcetate Substrate Mix, lyophilized, 1x vial\nProbe, lyophilized, 1x vial\nAcetate Standard, lyophilized, 1x vial\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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