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Acetyl CoA Carboxylase 1 (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

Cat no: A0224-01

Acetyl CoA Carboxylase 1 (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol. ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric interactions. Nutrients and hormones can be both short-term (inducing reversible phosphorylations by such as AMPK) and long-term (transcription level regulation) regulators of ACC1. Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland. ACC1 has been implicated as a target in the development of anti-obesity drugs.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:2000\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A0224-01

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Jianqiang M, et al. PNAS 100:7515-7520, 2003 2. Girad J, et al. The FASEB Journal 8:36-42, 1994 3. Wakil SJ, et al. Annu Rev Biochem 52:537

Additional Info

Recognizes human Acetyl-CoA carboxylase 1. \nSpecies Crossreactivity: mouse and rat

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