Acetyl CoA Carboxylase 1 (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCalpha (ACC1) is the predominant isoform found in liver, adipocytes and mammary gland, while the 280kD ACCbeta (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4). \n\nApplications:\nSuitable for use in Western Blot, Immunofluorescence, and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. \nImmunofluorescence (IC): 1:800\nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0224-02D

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Ruderman, N.B. et al. (1999) Am. J. Physiol. 276, E1-E18, 2. Ha, J. et al. (1994) J. Biol. Chem. 269, 22162-22168, 3. Abu-Elheiga, L. et al. (2001) Science 291, 2613-2616, 4. Levert, K.L. et al. (2002) J. Biol. Chem. 277.

Additional Info

Recongnizes endogenous levels of human total Acetyl CoA Carboxylase 1 protein. Species crossreactivity: mouse and rat.