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Acetyl CoA Carboxylase 1 Phosphorylated (Ser79) (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

Cat no: A0224-02B

Acetyl CoA Carboxylase 1 Phosphorylated (Ser79) (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

Acetyl-CoA carboxylase 1 (ACC1) is a biotin dependent lipogenic enzyme that is highly expressed during adipogenesis. ACC1 catalyzes acetyl-CoA carboxylation, producing malonyl-CoA, a metabolite involved in energy homeostasis regulation. Malonyl-CoA is a two carbon donor in the synthesis of long-chain fatty acids and the elongation of fatty acids found in the cystol (1). ACC1 is regulated short-term by citrate, CoA, and palmitoyl-CoA through allosteric \ninteractions. Nutrients and hormones can be both short-term inducing reversible by such as AMPK at Ser79 and others) and long-term (transcription level regulation) regulators of ACC1 (2). Highly expressed in lipogenic tissues, ACC1 is found in liver, adipose, and lactating mammary gland (3). ACC1 has been implicated as a target in the development of anti-obesity drugs (4). \n \nApplications: \nSuitable for use in Western Blot, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:5000 -10,000 \nImmunocytochemistry 1:100-250 \nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A0224-02B

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Serum

Isotype

IgG

References

1. Jianqiang M, et al. PNAS 100: 7515-7520, 2003. 2. Girad J, et al. The FASEB Journal 8: 36-42, 1994. \n3. Wakil SJ, et al. Annu Rev Biochem 52: 537

Additional Info

Recognizes human ACC1 phosphorylated at serine 79.

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