Acetylcholine Assay Kit, BioAssay(TM)

The United States Biological Acetylcholine Assay Kit provides a simple, direct and high throughput assay for measuring acetylcholine in biological samples. \n\nPrinciple of the Assay:\nIn this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585nm) is directly proportional to the acetylcholine concentration in the sample.\n\nApplications:\nAssays: Determination of acetylcholine in biological samples such as serum, plasma, urine, saliva, milk, tissue and cell culture.\nDrug Discovery/Pharmacology: Investigation of effects of drugs on acetylcholine metabolism.\n\nSample Size: 20ul\n\nLinear Detection Range: 100-200uM (fluorimetric assay); 0.4-10uM (colorimetric assay)\n\nKit Components:\nA0526-32A1: ACHE Enzyme, 1x120ul\nA0526-32A2: Enzyme Mix, 1x120ul\nA0526-32A3: Acetylcholine Standard (2mM), 1x400ul\nA0526-32A4: Dye Reagent, 1x120ul\nA0526-32A5: Assay Buffer, 1x10ml\n\nStorage and Stability:\nStore all components at -20 degrees C. Kit is stable for up to 3 months upon receipt.

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SPECIFICATIONS

Catalog Number

A0526-32A

Size

1Kit

References

1. Vizi, E.S. et al (1985). A simple and sensitive method of acetylcholine identification and assay. Bioassay combined with minicolumn gel filtration or high-performance liquid chromatography. J Pharmacol Methods. 13:201-211. 2. Gilberstadt, M.L. and Russell, J.A. (1984). Determination of picomole quantities of acetylcholine and acetylcholine in physiologic salt solutions. Anal Biochem. 138:78-85. 3. Israel, M. and Lesbats, B. (1982). Application to mammalian tissues of the chemiluminescent method for detecting acetylcholine. J Neurochem. 39:248-250.