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Acetylcholine, Conjugated

Cat no: A0526-31

Acetylcholine, Conjugated

Applications: \nSuitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000-1:5000.\nOptimal dilutions to be determined by the researcher.\n\nCrossreactivity Data:\nUsing a conjugate Choline-GA-Protein, antibodies specificity was performed with an ELISA test by competion experiments with the following compounds.\n\nCompound Crossreactivity ratio (a)\nCholine-GA-PL

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SPECIFICATIONS

Catalog Number

A0526-31

Size

100ug

Applications

ELISA, ICC, IHC

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, before the addition of glycerol to 40%. No preservative added.

P Type

Pab

Purity

Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.

Isotype

IgG

References

1. GEFFARD, M., VIEILLEMARINGE, J., HEINRICH-ROCK, A.M., DURIS, P.: Anti-acetylcholine antibodies and first immunocytochemical application in insect brain. Neurosci. Lett. 57: 1-6 (1985). 2. GEFFARD, M., McRAE-DEGUEURCE, A., SOUAN, M.L.: Immunocytochemical detection of acetylcholine in the rat central nervous system. Science 229: 77-79 (1985). 3. McRAE-DEGUEURCE, A., GEFFARD, M.: One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain. Brain Res. 376: 217-219 (1986). 4. WANG-BENNETT, L.T., SOUAN, M.L., GLANTZ, R.M.: Immunocytochemical studies of the distribution of acetylcholine in the crayfish. Brain J. of Comparative Neurol. 273: 330-343 (1988).

Additional Info

Recognizes protein conjugated Acetylcholine. See additional crossreactivity data.

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