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Acetylcholine Receptor (Muscarinic 1)

Cat no: A0526-40

Acetylcholine Receptor (Muscarinic 1)

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that mediates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m1 receptor is localized primarily in brain tissue. \n\nApplications: \nSuitable for use in Western Blot. Not suitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 2ug/ml. Detects an ~64kD protein representing recombinant mouse m1AChR.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0526-40

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ladner, C.J. & Lee, J.M., Exp. Neurol. 158: 451-458 (1999). 2. Wang, P., et al., J. Pharmacol. Exp. Ther. 273: 959-966 (1995).

Additional Info

Recognizes the Acetylcholine Receptor (Muscarinic 1). Species Crossreactivity: mouse

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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