Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft. \n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Immunoprecipitation. Not suitable for Western Blot and FACS.\nOther applications have not been tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 1:50. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0526-42D

Size

200ul

Applications

ELISA, IHC, IP

Hosts

Mouse

Reactivities

Hum, Bov, Fel, GP, Rabbit

Form

Supplied as a liquid in PBS with 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

References

1.General: This antibody has been used in: Cellular and Molecular Neurobiology, 8(1): 85-93, 1988. 2. General:Brain Research, 539: 233-238, 1991.J. Neural. Transm., 34: 139-1450, 1991.

Additional Info

Recognizes human Acetylcholinesterase. Does not detect butyrylcholinesterase (BChE). Cannot be used in Western blot to detect AChE. Species Crossreactivity: rabbit, bovine, feline and guinea pig.