ACHE, CT (Acetylcholinesterase)

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.\n\nApplications:\nSuitable for use in Western Blot, ELISA.\n\nRecommended Dilution: \nELISA: 1:1,000\nWestern Blot: 1:200~3200\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

030736

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, crude ascites with 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)\nHoward, T.D., et al. Environ. Health Perspect. 118(10):1395-1399(2010)\nCadieux, C.L., et al. Chem. Biol. Interact. 187 (1-3), 229-233 (2010)\nRuano, G., et al. Pharmacogenomics 11(7):959-971(2010)\nOhno, K., et al. Proc. Natl. Acad. Sci. U.S.A. 95(16):9654-9659(1998)

Additional Info

Human, hamster

Alternative Names

ACHE, Acetylcholinesterase