Acid Phosphatase Fluorometric BioAssay(TM) Kit

Acid phosphatase (AP) dephosphorylates phosphate groups from the phosphate esters in acid conditions. Different forms of acid phosphatase are found in different organs, and their serum levels are used as a diagnosis for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate resistant acid phosphatase levels may indicate the bone disease. The Acid Phosphatase Fluorometric Assay Kit uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTSready fluorometric assay designed to measure AP activity in serum and other biosamples. The detection sensitivity is ~1uU, more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.\n\nKit Components:\nAP *Assay Buffer 1x100ml\nMUP Substrate 1x1vial\nAP Enzyme 1x1vial\nStop Solution 1x25ml.\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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