Acid Sensing Ion Channels 4 (ASIC4)

Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+ -gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.\n\nThe mammalian homolog of degenerins (MDEG or MDEG1; now designated ASICs for Acid Sensing Ion Channels). Three are at least 4 distinct proteins in ASIC family ASIC1-4. AISC4/SPASIC (human 539/666aa, chromosome 2; rat 539aa, ~97% homology between rat and human) is strongly expressed in pituitary and also detected in most areas of brain. ASIC4 is the most divergent member of ASIC family showing only 45% identity with ASIC1-3. ASIC4 is not activated by low pH. It may associate with other ASICs to form a functional channel.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 \nELISA: 1:10,000-50,000 \n\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: A0577-10\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0577

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid, serum, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Akopian, A.N., et al. (2000) Neuroprot. 14: 2217-2222. 2. Grunder, S., et al. (2000) Neuroprot. 14: 1607-1611.

Additional Info

Recognizes Acid Sensing Ion Channels 4. No significant sequence homology is detected with other ASIC-beta or other ASICs. Species Sequence Homology: Rat: 100%.