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Acidic Hair Keratin, K34 (KRT34, Keratin 34, HA4, Ha-4, Hair Keratin, Type I Ha4, hHa4, HHA4, HKA4, K34, Keratin, Type I Cuticular Ha4, Keratin-34, KRTHA4 hHa4)

Cat no: A0578-81

Acidic Hair Keratin, K34 (KRT34, Keratin 34, HA4, Ha-4, Hair Keratin, Type I Ha4, hHa4, HHA4, HKA4, K34, Keratin, Type I Cuticular Ha4, Keratin-34, KRTHA4 hHa4)

Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, it is recommended to preincubate the fixed sections with 0.1% Triton X-100 in PBS for 1-5 minutes prior to first incubation step. Incubation time: 1 hour at RT\nWestern Blot (ECL): 1:2000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0578-81

Size

100ul

Applications

IHC, WB

Reactivities

Hum, Mouse

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Langbein, L., et al., J. Biol. Chem. 274: 19,874-19,893 (1999). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004).

Additional Info

Recognizes human hair keratin K34 (hHa4) expressed in the upper hair cortex cells. Species Crossreactivity: mouse.

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