Acidic Hair Keratin, K37 (KRT37, Keratin 37 HA7, Hair keratin, type I Ha7, hHa7, HHA7, HKA7, K37, Keratin, type I cuticular Ha7, Keratin-37, KRTHA7, hHa7)

Type I hair keratin is one of the two types of hair keratin. It is an acidic protein which heterodimerizes with type II hair keratins to form hair and nails.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1:200. For enhancement of cortex staining, preincubate fixed sections with Triton X-100 (in PBS) for 1-5 minutes prior to first antibody incubation step.\nWestern Blot (ECL): 1:2000 Incubate for 1 hour at RT.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0578-87

Size

100ul

Applications

IHC, WB

Reactivities

Hum

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1.Langbein L, Rogers M, Winter H, Pra?tzel S, Beckhaus U, Rackwitz HR, Schweizer J: The catalog of human hair keratins: I. Expres- sion of nine type I members in the hair follicle. J Biol Chem 274, 19874-19893 (1999)2.Jave-Suarez LF, Langbein L, Winter H, Praetzel S, Rogers ML, Schweizer J : Androgen regulation of the human hair follicle : The type I hair keratin hHa7 is a direct target gene in trichocytes.3. J Invest Dermatol 122, 555-564 (2004)Langbein L, Spring H, Rogers MA, Praetzel S, Schweizer J: Hair keratins and hair follicle-specific epithelial keratins. Methods in Cell Biology 78, 413-456 (2004)

Additional Info

Recognizes human hair keratin K37 expressed starting from the middle cortex of vellous hairs. Negative with cells in the anagen hair follicle. Species Crossreactivity: chimpanzee