Acinus (AAE7, ACN1, AC NON-UTILIZING 1, ACYL-ACTIVATING ENZYME 7, AT3G16910.1, Apoptotic Chromatin condensation inducer in nucleus)

Chromatin condensation and nuclear fragmentation (CCNF) are the hallmarks of apoptosis. CCNF is triggered by the activation of members of the caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by Caspase 3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated Acinus L (1341 amino acids), Acinus S (583 amino acids) and Acinus S' (614 amino acids).\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nOptimal working dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0600-01D

Size

100ug

Applications

WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS with 0.02% sodium azide.

P Type

Pab

Purity

Purified IgG was prepared from whole serum by affinity chromatography.

Isotype

IgG

References

Sahara, S. et al. (1999). Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature. 401: 168-73

Additional Info

Recognizes Acinus (apoptosis chromatin condensation inducer in the nucleus), an inducer of chromatin condensation. The antibody recognizes a C-terminal region within three splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'.