Acrosin (Acrosin precursor, ACRS, ACR-2)

ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0700-02A

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Prc

Form

Supplied as a liquid in PBS, pH 7.4, containing 15mM sodium azide

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

This product has been used in:Moos J et al. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993). <PUBMED:8373968>Peknicova J & Moos J Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia 22:427-35 (1990). <PUBMED:2073053>

Additional Info

Reacts with various forms of porcine acrosin (55, 53, 45 and 35kD) in the acrosome of undamaged spermatozoa. Does not crossreact with bovine, canine or human. Not yet tested in other species.