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Acrosin (Acrosin Precursor, ACRS, ACR, 53kD Fucose-binding Protein)

Cat no: A0700-75

Acrosin (Acrosin Precursor, ACRS, ACR, 53kD Fucose-binding Protein)

ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.\n\nApplications:\nSuitable for use in Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 1:10 Membrane permeabilization with acetone is essential.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0700-75

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Prc

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Peknicova, P., and Moos, J., 1990, Andrologica 22, 427-435. 2. Moos J. et al., 1993 Biol. Reprod. 49, 408. 3. Peknicova, P., et al., 1994, Anim. Reprod. Sci, 35, 255.

Additional Info

Recognizes various forms of porcine acrosin (55, 53, 45 and 35kD) in the acrosome of undamaged spermatozoa. Does not cross react with human, bull or canine sperms.

Alternative Names

EC=3.4.21.10

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