Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including Acrp30, involved in lipid metabolism and glucose homeostasis. Acrp30 (Adipocyte complement-related protein of 30kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulinresistance. Treatment of db/db mice with TZD increased Acrp30 levels. Acrp30 (mouse 247aa, rat human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa), followed by a 27aa unique region, and then by 22 perfect Gly-XPro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus. Structurally, but at the sequence level, Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins). Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. A marked reduction in plasma triglycerides, glucose, and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle. Full length Acrp30 was less potent than gAcrp30. Therefore, gAcrp30 may open new avenues to control obesity.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-50,000; 10-100ng of control peptide/well\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0725-02V

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Scherer PE et al (1995) JBC 270, 26746. 2. Hu E et al (1996) JBC 271, 10697. 3. Das K et al 2001) BBRC 280, 1120. 4. Fruebis J et al (2001) PNAS 98, 2005. 5. Maeda K et al (1996) BBRC 221, 286. 6. Schaffler A et al (1998) BBA 1399, 187. 7. Schaffler A et al (1999) BBRC 260, 416.

Additional Info

Recognizes human Acrp30.