Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28) (Biotin)

Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, and Adipocyte complement related protein of 30kD (Acrp 30). Acrp30 is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Acrp 30 have been shown to be induced over 100-fold. Studies indicate that Acrp30 enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Acrp30 function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Acrp30 secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Acrp30 have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNF-alpha suggests that Acrp30 may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Acrp30 is down-regulated in various forms of obesity and its structural similarity to TNF-alpha, it is currently being investigated as an important regulator of whole body energy homeostasis. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nELISA (direct and indirect): 1:5000-1:10,000\nWestern Blot: 1:5000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; Incubate 1 hour at RT.\nOptimal conditions to be determined by the researcher.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0725-15A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

P Type

Pab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG

References

1.Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)

Additional Info

Recognizes human adiponectin at ~30kD.