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Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28) (Biotin)

Cat no: A0725-16A

Acrp30 (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28) (Biotin)

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks of energy balance, adipocytes express many genes, including Acrp30 which is involved in lipid metabolism and glucose homeostasis.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nELISA (Direct and Indirect): 1:5000-1:10,000\nWestern Blot: 1:5000-1:10,000 using ECL; Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0725-16A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4. Labeled with biotin. Contains no preservatives.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes both natural and recombinant mouse adiponectin. Detects a band of ~35kD by Western blot. Species Crossreactivity: rat (weak).

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