Acrp30, CT (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Acrp30 (adipocyte complement-related protein of 30 kD), also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28 kD/GBP28 or adipocyte most abundant gene transcript) was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Acrp30 (mouse 247 aa, rat human 244 aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14 aa), followed by a 27-aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus. Acrp30 is proteolytically cleaved at 104 aa to generate the globular Acrp30 (gAcrp30). Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml (ECL).\nELISA: 1:5000-25,000. Coat plate with 10-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: A0725-03\n\nMolecular Weights:\nFull length Acrp30: ~30kD.\nRecombinant Acrp30: ~37kD\ngAcrp30:~16kD.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0725-04

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Scherer, P.E., et al. (1995) JBC 270: 26746. 2. Hu, E., et al. (1996) JBC 271: 10697. 3. Das, K., et al. (2001) BBRC 280: 1120. 4. Fruebis, J., et al. (2001) PNAS 98: 2005. 5. Maeda, K., et al. (1996) BBRC 221: 286. 6. Schaffler, A., et al. (1998) BBA 1399: 187. 7. Schaffler, A., et al. (1999) BBRC 260: 416.

Additional Info

Recognizes mouse Acrp30. Detects both the globular (truncated) and full length Acrp30 proteins because the epitope is located near the C-terminus. No significant sequence similarity with the related protein C1q. \nSpecies Sequence Homology: Bovine Acrp30: 100%; human and monkey Acrp30: 90%.