Acrp30, glycosylated, Recombinant, Human (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. \nActivity in vitro:\nIn vitro gluconeogenesis assay in primary hepatocytes was performed, showing the human adiponectin derived from mammalian cells can inhibit glucose production. The ED50 was ~6ug/ml. Effect of human adiponectin on gluconeogenesis in primary rat hepatocytes. Primary rat hepatocytes were isolated and maintained as described by Wang et al., 2002. The cells were then treated with 50 pM insulin plus different concentration of human adiponectin purified from HEK293 cells for 48 hours. At the end of treatment, the cells were incubated with a glucose-free medium for 6 hours, and glucose production was measured. Effect of recombinant human adiponectin on macrophage scanvager receptor A (SRA) expression in THP1-derived macrophages. THP-1 cells grown in 6-well dishes were differentiated into macrophages using PMA. After startvation in 0.5% FBS culture medium for 16 hours, the cells were treated with 5ug/ml human recombinant adiponectin for 2 and 3 days, respectively. Total RNA was purified using Trizol reagents. The mRNA abundance of SRA was quantified using quantitative RT PCR (Celera Gene ID: hCG27149), and normalized against 18S RNA (n=4). \n\nApplications:\nSuitable for use in ELISA, Western Blot, cell culture and/or animal studies Effect of human AdN on scanvager receptor A expression. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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