Acrp30, Recombinant, Human (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (adipokine). The protein belongs to the soluble defense collagen superfamily; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. \nThis human Adiponectin is created as a recombinant protein with N-terminal fusion of HisTag. The human Adiponectin His-Tagged fusion protein, produced in E. coli, is a 26.4kD protein containing 230 amino acid residues of the human Adiponectin and 12 additional amino acid residues-HisTag (underlined). \nThe aa sequence of A0725-50D is 100% homologous to the aa sequence of human serum adiponectin.\n\nAA Sequence:\nMRGSHHHHHH GSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY VTIPNMPIRF TKIFYNQQNH YDGSTGKFHC NIPGLYYFAY HITVYMKDVK VSLFKKDKAM LFTYDQYQEN NVDQASGSVL LHLEVGDQVW LQVYGEGERN GLYADNDNDS TFTGFLLYHD TN\n\nQuality Control Test:\nBCA-to determine content of the protein. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0725-50D

Size

10ug

Applications

WB

Form

Supplied as a lyophilized powder from 0.02M Tris buffer, pH 7.5, 0.15M sodium chloride. Reconsitute with 200ul of 0.1M acetate buffer, pH 4, and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited.

Purity

Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding. > 95% (SDS-PAGE analyzed). SDS-PAGE 12% separation of human adiponectin 5ug/ line.

References

1. Xu, A., et al., J. Clin. Invest. 112: 91-100 (2003). 2. Wang, Y., et al., J. Biol. Chem. 277: 19,521-19,529 (2002). 3. Yamauchi, T., et al., Nat. Med. 8: 1288-1295 (2002). 4. Berg, A.H., et al., Nat. Med. 7: 947-953 (2001).