Acrp30, Recombinant, Mouse (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kD and exclusively expressed in differentiated adipocytes. Adiponectin(Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain(gAcrp30). Adiponectin(Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30(247aa) or gAcrp30(globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30(amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques. \n\nSequence:\nMAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITVYMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYADNVNDSTFTG FLLYHDTN \n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Recombinant Acrp30. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A0725-03L

Size

20ug

Form

Supplied as a liquid in 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT and 10% Glycerol.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Serum adiponectin levels are an independent predictor of the extent of coronary artery disease in men. J Am Coll Cardiol 2006 May 16;47(10):2124-6 2. The effect of liposuction and diet on ghrelin, adiponectin, and leptin levels in obese Zucker rats. Plast Reconstr Surg 2006 May;117(6):1829-35 3. Adiponectin replenishment ameliorates obesity-related hypertension. Hypertension 2006 Jun;47(6):1108-16 4. The association of plasma adiponectin level with carotid arterial stiffness. Metabolism 2006 May;55(5):587-92 5. Body fat at birth and cord blood levels of insulin, adiponectin, leptin, and insulin-like growth factor-I in small-for-gestational-age infants. Arch Med Res 2006 May;37(4):490-4 6. Adiponectin and risk of congestive heart failure JAMA 2006 Apr 19;295(15):1772-4