Acrp30, Trimeric Form, Recombinant, Mouse (Adipocyte Complement-related Protein of 30kD, Adiponectin, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28)

Adiponectin (also called ACRP30, GBP28 and apM1) is a hormone exclusively expressed from adipose tissue 1. Many studies demonstrate that adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. Adiponectin can increase insulin sensitivity of skeletal muscle 2, attenuate hepatic lipogenesis 3 and luconeogenesis 4, regulate NO production in endothelial cells 5, inhibit proliefeartion of smooth muscle cells 6 and prevent lipid accumulation of macrophage cells 7. \nIn the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (MMW, also called trimer) forms 8. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions. \nTrimeric form of adiponectin was expressed in HEK293 cells stably transfected with pcmAd-F vector encoding FLAG epitope-tagged mouse adiponectin. The cystenine 39 was replaced with alanine (C39A). mAd-C39A can only form trimer, but not hexamer or HMW form. Recombinant mouse adiponectin

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SPECIFICATIONS

Catalog Number

A0725-66

Size

2ug

Form

Supplied as a powder lyophilized from solution containing PBS, pH 7.2. \nReconstitution: Add deionized water to a concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile; sterile-filter solution before using it in cell culture.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Wang Y, Lam KS, Xu JY, Lu G, Xu LY, Cooper GJ, Xu A. Adiponectin inhibits cell proliferation by interacting with several growth factors in an oligomerization-dependent manner. J Biol Chem. 2005 May 6;280(18):18341-7. 2. Berg AH, Combs TP, Scherer PE. ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism. Trends Endocrinol Metab. 2002;13:84-89. 3. Yamauchi T, Kamon J, et al. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med. 2001;7:941-946. 4. Xu A, Wang Y, Keshaw H, Xu LY, Lam KS, Cooper GJ. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice. J Clin Invest. 2003;112:91-100. 5. Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med. 2001;7:947-953. 6. Chen H, Montagnani M, Funahashi T, Shimomura I, Quon MJ. Adiponectin stimulates production of nitric oxide in vascular endothelial cells. J Biol Chem. 2003;278:45021-45026. 7. Matsuda M, Shimomura I, et al. Role of adiponectin in preventing vascular stenosis. The missing link of adipo-vascular axis. J Biol Chem. 2002;277:37487-37491. 8. Okamoto Y, Kihara S, et al. Adiponectin reduces atherosclerosis in apolipoprotein E-deficient mice. Circulation. 2002;106:2767-2770. 9. Kobayashi H, Ouchi N, Kihara S, Walsh K, Kumada M, Abe Y, Funahashi T, Matsuzawa Y, Ferre P. Selective Suppression of Endothelial Cell Apoptosis by the High Molecular Weight