ACTB (Actin, Cytoplasmic 1, Beta-actin) (HRP)

In vertebrates 3 main groups of actin isoforms, A, B and G have been identified. The A-actins are found in muscle tissues and are a major constituent of the contractile apparatus. The B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. B-actins are highly conserved proteins that are involved in cell motility, structure and integrity. B-actins are cytoplasmic proteins.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:20,000\nWestern Blot: 1:1000-1:5000\nImmunohistochemistry: 1:500-1:2500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

168007

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Goat, GP, NHP, Prc, Sheep

Form

Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human B-actin. Species Crossreactivity: Mouse, rat, monkey, sheep, bovine, goat, guinea pig, porcine and hamster.