Actin

Actin is an abundant cytoskeletal protein found in all cells. The protein

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SPECIFICATIONS

Catalog Number

A0759-07

Size

100ug

Applications

ICC, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b,k

References

1. Jazirehi, A.R., et al., Cancer Res. 65: 264-276 (2005). 2. Tsou, T., et al., Chem. Res. Toxicol. 17: 208-217 (2004). 3. Jazirehi, A.R., et al., Cancer Res. 64: 7117-7126 (2004). 4. Jazirehi, A.R., et al., Mol. Cancer Ther. 3: 71-84 (2004). 5. Navarro, C.L., et al., Hum. Mol. Genet. 13: 2493-2503 (2004). 6. Luciano, L., et al., J. Histochem. Cytochem. 51(2): 187-198 (2003). 7. True, L.D., Atlas of Diag. Immunopat. Gower Medical Pub., N.Y: 6.5 (1990). 8. Lessard, J., Cell Motility and the Cytoskeleton. 10: 349-362 (1988). 9. Otey, C., et al., J. Cell. Biol. 102: 1726-1737 (1986).

Additional Info

Recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species Crossreactivity: All vertebrates as well as Dictyostelium discoideum and Physarum polycephalum.