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Actin

Cat no: A0759-08A

Actin

Applications:\nSuitable for use in Immunocytochemistry, Immunoblot, Immuofluorescence and Immunohistochemistry (frozen tissue).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nMuscle or myofibrils\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0759-08A

Size

1ml

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird

Form

Supplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Culture supernatant

Isotype

IgM

References

Kaehn K, Bachmann P, Falkenberg FW. Immunofluorescence Staining of Thin-filament sections not Participating in Actomyosin Crossbridges: Studies by Use of a Monoclonal Antibody to Actin. Cell Tissue Res 239, 417-422 (1985)

Additional Info

Useful for studying the intracellular distribution and structure of actin in the cytoskeletal system. In immunoblotting one single band corresponding to actin. Species Crossreactivity: Human, mouse, rat, chicken.

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