Actin

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex. It has been reported that actin is hyperphosphorylated in primary breast tumors. It has also been shown that actin dysfunction leads to certain types of heart failure.\n\nApplications: \nSuitable for use in Indirect Immunofluorescence, ELISA, Western Blot Dot Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nIndirect Immunofluorescence: 1:200-1:2000. Staining of cultured human or chicken fibroblasts\nWestern Blot: 1:500 using Human or chicken fibroblast extract. Band at ~ 42kD. \nImmunohistochemistry (Paraffin): Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solution can be used as fixatives. Can also be used on frozen sections.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0759-08M

Size

200ul

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Goat, GP, Prc, Rabbit, Sheep

Form

Supplied as a liquid, 15mM sodium azide

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

1. Herman, I., Curr. Opin. Cell Biol., 5, 48 (1993). 2. Vanderkerckhove, J., and Weber, K., Eur. J. Biochem., 90, 451 (1978). 3. Drew, J., et al., Amer. J. Physiol., 260, C1332 (1991). 4. Lessard, J., Cell Motil. Cytoskel., 10, 349 (1988). 5. Sutoh, K., and Mabuchi, I., Biochemistry, 25, 6186 (1986).

Additional Info

Recognizes human actin. Species Crossreactivity: mouse, rat, bovine, chicken, canine, goat, guinea pig, porcine, rabbit, amphibian and sheep Does not react with the epitope on the N-terminal end of actin.

Alternative Names

ActG, ActG1, DFNA20