Actin alpha 2, Smooth Muscle (ACTa2) BioAssay(TM) ELISA Kit (Human)

Actin Alpha 2, Smooth Muscle (ACTa2) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACTa2 in human serum, plasma and other biological fluids.\n\nDetection Range:\n7.813-500ng/ml\n\nSensitivity:\n~1.7ng/ml\n\nIntra-Assay: \nCV<10%\n\nInter-Assay: \nCV<12%\n\nKit Components:\n*023092A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use\n*023092B: Standard, 2x1vialпїЅ\n023092C: Standard Diluent, 1x20ml\n*023092D: Detection Reagent A, 1x120ulпїЅ\n*023092: Detection Reagent B, 1x120ulпїЅ\n023092F: Assay Diluent A, 2x, 1x6ml\n023092G: Assay Diluent B, 2x, 1x6ml\n023092H: TMB Substrate, 1x9ml\n023092J: Positive Control, 28.69ng/ml, 1x1vial\n023092K: Stop Solution, 1x6ml\n023092L: Wash Buffer, 30X, 1x20ml\n\nPrecaution:\nThe Stop Solution (026229K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.\n\nNotes on 023092J - Positive Control:\nIntended Use:\nThe control vials are from human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.\n\nCharacteristics:\nThe control vials contain human ACTa2 in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.\n\nExpected Values:\n28.69ng/ml\nThe concentration of the control was determined by subtracting two standard deviations to the mean optical density value of twenty QC sample replicates and calculating the corresponding concentration.\n\nUsage:\nDisslove with 150ul standard dilutent and let stand for 10min. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4 degrees C and use within 5 days.\n\nStorage and Stability:\nStore *023092A, *023092B, *023092D and *023092E at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

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