Actin, alpha (ACTA1, ACTA, skeletal muscle, Alpha-actin-1)

Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000\nImmunohistochemistry (Paraffin): 10ug/ml\nImmunohistochemistry (Frozen): 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

135880

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Goat, Prc, Rabbit

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

Additional Info

Recognizes human ACTA1/ASMA.Highly specific for alpha- skeletal actin, and does not cross react with other actin isoforms. The epitope recognized by 3B3 is highly conserved. Therefore cross-reacts with many other species. Species Crossreactivity: Goat, porcine, rabbit, rat.