Actin, alpha, Smooth Muscle

Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells.\n\nApplications: \nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nImmunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSmooth muscle or leiomyosarcoma\n\nSource: \nAscites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-26

Size

500ul

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See A0760-26X.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Skalli, O., et al., Journal of Cell Biology 103: 2787-2796 (1986). 2. Bochaton-Piallat, M.L., et al., Journal of Submicroscopic Cytology and Pathology 26: 1-8 (1994). 3. Cremona, O., et al., Journal of Histochemistry and Cytochemistry 42: 1221-1228 (1994). 4. Estes, J.M., Differentiation 56: 173-181 (1994). 5. Orlandi, A., et al., Arteriosclerosis and Thrombosis 14: 982-989 (1994).

Additional Info

Recognizes human alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells. Species Crossreactivity: baboon, bovine, rabbit, mouse, rat and chicken