Actin, alpha, Smooth Muscle

Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues.\n\nApplications: \nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml Human smooth muscle actin has a predicted length of 377 residues and a MW of 42kD.\nImmunohistochemistry (formalin fixed and paraffin): 0.2-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, pH 7.4, to concentration of 0.1-1mg/ml.. Reconstituted product is stable for 12 months at -20 degrees C. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-27E

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Prc, Rabbit

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.02% sodium azide. Reconstitute in 1ml of PBS to concentration of 0.1-1mg/ml.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins. Species crossreactivity: Human, mouse, porcine, baboon, rabbit.

Alternative Names

ACTA2, ACTSA, ACTVS