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Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)

Cat no: A0760-26B

Actin, alpha, Smooth Muscle (Actin, Aortic Smooth Muscle, Alpha-actin-2, Cell Growth-inhibiting Gene 46 Protein, ACTA2, ACTSA, ACTVS, GIG46)

Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24. Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 2ug/ml\nImmunohistochemistry (Formalin or acetone fixed): 0.3-1.2ug/ml \nImmunohistochemistry: Formalin fixed paraffin embedded\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1.2% sodium acetate or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-26B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1ml 1.2% sodium acetate or PBS.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2a

References

1. Kumar, MS. et al., (2003). Circ. Res. 92: 840-847. 2. Ueyama, H. et al. (1990). Jpn. J. Hum. Genet. 35: 145-150.

Additional Info

Recognizes human Smooth Muscle Actin, alpha. Species Crossreactivity: mouse and rat

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