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Actin, alpha, Smooth Muscle (PE)

Cat no: A0760-27F

Actin, alpha, Smooth Muscle (PE)

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. 10ul labels up to 1x10e6 cells.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nFusion of a mouse myeloma with B cells obtained from a rat immunized with a synthetic decapeptide from the N-terminal of Alpha-Smooth Muscle Actin.\n \nStorage and Stability:\nMaybe stored at 4 degrees C before opening. DO NOT FREEZE! Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A0760-27F

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Rabbit

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG2a

References

1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787. 2. Oishi, K. et al. (2002) J. Physiol. 540:139.

Additional Info

Recognizes human Alpha-Smooth Muscle Actin. Species Crossreactivity: rabbit, mouse and rat.

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