Actin, beta

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.\n\nApplications: \nSuitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control.\nImmunofluorescence: 1:50.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa whole cell lysate and 3T3 cells (mouse).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-40A

Size

50ul

Applications

ICC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in Tris-citrate, phosphate buffer, pH 7.0, 0.09% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

US Biological application reference: Jammart, B. et al., J. Virol. (2013) 87; 5065-5080. 1. Zipris, D., et al., J. Immunol. 174: 131-42 (2005). 2. Gardiner, T.A., et al., Am. J. Pathol. 166: 637-44 (2005). 3. Conti, B.J., et al., J. Biol. Chem. 280: 18,375-18,385 (2005). 4. Andreadi, C.K, et al., Mol. Pharmacol. 69: 1033-1040 (2006). 5. Auten, R.L., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 290: L32-L40 (2006). 6. Mikhailik, A., et al., Front. Biosci. 11: 883-888 (2006).

Additional Info

Recognizes human beta Actin. Species Crossreactivity: mouse, rat, monkey. Expected to crossreact with a wide range of species due to sequence homology.