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Actin, beta

Cat no: A0760-40D

Actin, beta

Beta actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissue tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to b-actin provides a useful and specific tool in the study of the intracellular distribution of b-actin and the static and dynamic aspects of the cytoskeleton.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1.0ug/ml\nWestern Blot: 1.0ug/ml\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0-AG14 mouse myeloma cells with spleen cells.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-40D

Size

40ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Bov, Ch/Bird, Goat, Prc, Rabbit

Form

Supplied as a lyophilized powder from PBS pH 7.4, 1% BSA, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

Additional Info

Recognizes beta Actin. Species Reactivity: mouse, rabbit, chicken, human, hamster, bovine, goat, fish and porcine.

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