Actin, beta

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one bactin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1: 50,00 Control peptide can be used to coat ELISA plates at 1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-41A

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute in 100ul PBS.

P Type

Pab

Purity

Serum.

Isotype

IgG

References

(1). Ohmuri H (1995) Gene Accession #S38782; Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451; Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349; North JA et al (1994) J. Cell Sci. 107, 437.

Additional Info

Recognizes human Beta-Actin. Species Crossreactivity: mouse and rat.