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Actin, beta

Actin, beta

Cat no: A0760-41A

Supplier: United States Biological
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Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one bactin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1: 50,00 Control peptide can be used to coat ELISA plates at 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0760-41A
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA
Size: 100ul
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute in 100ul PBS.
P type: Pab
Isotype: IgG
Purity: Serum.
References: (1). Ohmuri H (1995) Gene Accession #S38782; Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451; Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349; North JA et al (1994) J. Cell Sci. 107, 437.
Additional info: Recognizes human Beta-Actin. Species Crossreactivity: mouse and rat.

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