Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

All eukaryotic cells express actin, which oftern constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, high eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha-actin expression is limited to various types of muscle, whereas beta- and gamma-actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion. Rac regulares actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-40P

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Lessard, J.L., Cytoskeleton 10: 349-362 (1988). 2. Doolittle, R.F., Philos, Trans. R. Soc. Lond., B, Biol. Sci. 349: 235-240 (1995). 3. Maccioni, R.B. & Cambiazo, V., Physiol. Rev. 75: 835-964 (1995). 4. Schutt, C.E., et al., J. Struct. Biol. 115: 186-198 (1995). 5. Barkalow, K. & Hartwig, J.H., Curr. Biol. 5: 1000-1002 (1995). 6. Nobes, C.D. & Hall, A., Cell 81: 53-62 (1995). 7. Graf, R., et al., Cell Tissue Res. 283: 133-141 (1996). 8. Furumura, M. & Ishikawa, H., Cell Tissue Res. 283: 425-434 (1996).

Additional Info

Recognizes endogenous levels of total human beta Actin. Species Crossreactivity: mouse, rat