Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

Cat no: A0760-40P

Supplier: United States Biological

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All eukaryotic cells express actin, which oftern constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, high eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. Alpha-actin expression is limited to various types of muscle, whereas beta- and gamma-actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion. Rac regulares actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	A0760-40P
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	ELISA, Western Blot
Size:	100ul
Form:	Supplied as a liquid.
P type:	Pab
Isotype:	IgG
Purity:	Purified by Immunoaffinity chromatography.
References:	1. Lessard, J.L., Cytoskeleton 10: 349-362 (1988). 2. Doolittle, R.F., Philos, Trans. R. Soc. Lond., B, Biol. Sci. 349: 235-240 (1995). 3. Maccioni, R.B. & Cambiazo, V., Physiol. Rev. 75: 835-964 (1995). 4. Schutt, C.E., et al., J. Struct. Biol. 115: 186-198 (1995). 5. Barkalow, K. & Hartwig, J.H., Curr. Biol. 5: 1000-1002 (1995). 6. Nobes, C.D. & Hall, A., Cell 81: 53-62 (1995). 7. Graf, R., et al., Cell Tissue Res. 283: 133-141 (1996). 8. Furumura, M. & Ishikawa, H., Cell Tissue Res. 283: 425-434 (1996).
Additional info:	Recognizes endogenous levels of total human beta Actin. Species Crossreactivity: mouse, rat

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Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

Cat no: A0760-40P

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