Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) (HRP)

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and g-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). a-cardiac anda-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and g-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A0760-41L

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP

Form

Supplied as a liquid in 136mM sodium chloride 2.6mM potassium chloride 12mM sodium phosphate, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).

P Type

Mab

Purity

Supernatant

Isotype

IgG

Additional Info

Recognizes endogenous levels of total beta-actin protein. May cross-react with gamma-actin (cytoplasmic isoform). Does not cross-react with alpha-skeletal, alpha-cardiac, alpha-vascular smooth muscle, or gamma-enteric smooth muscle isoforms. Species crossreactivity: Mouse, rat, monkey, bovine, and porcine.