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Actin, beta, CT (ACTB, Actin, Cytoplasmic 1, Beta-actin)

Actin, beta, CT (ACTB, Actin, Cytoplasmic 1, Beta-actin)

Cat no: A0760-41V

Supplier: United States Biological
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Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms, which have been identified. Like GAPDH, beta actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000 Immunohistochemistry: 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0760-41V
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Lambrechts A, et al (2004) Int. J. Biochem. Cell Biol. 36:1890-909. 2. Gourlay CW, et al (2005) Nat. Rev. 6:583-9.
Additional info: Recognizes human beta-actin. Species Crossreactivity: rat and mouse.

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