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Actin, beta (FITC)

Cat no: A0760-39B


Supplier: United States Biological
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Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern Blots. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 Western Blot: 1:1500-1:2000 Immunofluorescence: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: A0760-39B
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
P type: Mab
Isotype: IgG
Purity: Purified.
References: 1. Ohmuri H (1995) Gene Accession #S38782; Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451; L2. essard J et al (1988) Cell. Motil Cytoskel. 10, 349; 3. North JA et al (1994) J. Cell Sci. 107, 437.
Additional info: Recognizes human Beta-Actin.

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