Actin, beta (HRP)

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle, one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:5000-1:10,000 \nWestern Blot: 1:1000-1:5000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A0760-43B

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% merthiolate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 100ul sterile PBS.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Ohmuri H (1995) Gene Accession #S38782. 2. Vandekerchove, J et al (1978) Eur. J. Biochem. 90, 451. 3. Lessard J et al (1988) Cell. Motil Cytoskel. 10, 349. 4. North JA et al (1994) J. Cell Sci. 107, 437.

Additional Info

Recognizes human beta Actin.