Actin, beta Human

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (gsmooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western blots.\n\nBiological activity: \nNot tested. If the protein is stored and used properly can be used for biological assay. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml Used to coat plates\nWestern Blot: 100-500ng/lane in SDS-PAGE sample buffer (reduced) and load as needed.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0760-44F

Size

100ug

Applications

ELISA

Reactivities

Hum

Form

Supplied as a lyophilized powder from 5mM Tris. pH 8, 0.2mM calcium chloride, 0.2mM ATP, 5% sucrose, 1% dextran. Reconstitute with 100ul ddH20.

Purity

Purified (~99%).

References

1. Ohmuri H (1995) Gene Accession # S38782 2. Vandekerchove, J et al (1978) Eur. J. Biochem. 90:451. 3. Lessard J et al (1988) Cell. Motil Cytoskel. 10:349. 4. North JA et al (1994) J. Cell Sci. 107:437.