Actin, gamma

Actin is a 43kD highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. There are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:500-1:2000. Non-specific bands can occur when A0760-45 is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kD band\nImmunocytochemistry: 1:500-1:2000. Cultured cells fixed with PFA, then permeabilized with methanol.\nImmunohistochemistry: 1:500-1:2000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-45

Size

100ul

Applications

ICC, IHC, WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes human gamma actin. Species Crossreactivity: rat and mouse. Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton.