Actin, Muscle

Muscle specific actin is a 42kD protein specific for actin present in skeletal, cardiac, and smooth muscle. This antibody may be useful in the study of tumors arising from these muscle cells, such as rhabdomyosarcomas, leiomyomas and leiomyosarcomas.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: Paraffin. 1:10-1:40, Frozen. Does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.\nClone 3H521 is reported to be suitable for use in Western blotting (1).\n\nOptimal working dilutions to be determined by researcher.\n\nRecommended positive control tissue: \nSkeletal tissue.\n\nRecommended Secondary Reagents: \nF(ab')2 rabbit anti-mouse IgG HRP\n\nRecommended Negative Controls: \nMouse IgG1 Negative Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0761-11

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird, Fel, NHP

Form

Tissue Culture Supernatant. Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Tissue culture supernatant.

Isotype

IgG1

References

1. Tsukada, T. et al. (1987). HHF35, a muscle actin specific monoclonal antibody. I. Immunocytochemical and biochemical characterisation. Am. J. Pathol. 126: 51 - 60. 2. Tsukuda, T. et al. (1987). HHF35, a muscle actin spec

Additional Info

Recognizes muscle specific actin. Clone 3H521 is specific for alpha actin and does not recognize beta actin. Species Crossreactivity: Clone 3H521 is reported to crossreact with canine (3), cavia (4), feline (5), lapine (6), Japanese macaque monkey (7), shrew (7), mouse (7), rat (7, 8), monkey (1) and chicken (9).